[Frequency of 35delG mutation in GJB2 gene in non-syndromic prelingual hearing loss in 3 provinces of Iran]

Hearing loss is the most common inherited sensory disorder. At least 50% of hearing loss is inherited and about half of the genetic hearing loss is autosomal recessive non-syndromic. Mutations in GJB2 gene is the most frequent cause of autosomal recessive non-syndromic hearing loss. A single 35delG mutation is the most common allelic variant of GJB2 in most parts of the world. The aim of this study was to determine the rate of 35delG mutation in non-syndromic prelingual hearing loss in 3 provinces of Iran. In this descriptive experimental study, 240 cases with autosomal recessive non-syndromic hearing loss in 3 provinces of Iran, including Azarbaijan Sharghi [97 cases], Chaharmahal va Bakhtiari [98 cases] and Gilan [45 cases] were screened for 35delG mutation in the GJB2 gene. Blood samples [5 ml] were taken for genomic DNA extraction. The mutation was screened using Nested-PCR method and the positive results were confirmed by subsequent direct sequencing. Results of this study showed that from 240 studied patients [480 chromosomes], 35delG mutation was found in 58 chromosomes [24 patients were homozygote and 10 patients were heterozygote]. The frequency of 35delG mutation was 12.08%, including 18.04% in Azarbaijan Sharghi, 3.06% in Chaharmahal va Bakhtiari and 18.88% in Gilan province. Prevalence of 35delG mutation in Chaharmahal va Bakhtiari population was lower than other provinces studied. These results indicate that the other genes or mutations could result in autosomal recessive non-syndromic hearing loss in Chaharmahal va Bakhtiari population. However, as we found a low rate of 35delG in the populations studied, the cause of deafness remains to be detected in other loci or genes

[The degree of unemployment and reasons for not finding appropriate jobs among Iranian physicians]

Unemployment which had been a problem of less educated and unskilled people has become a problem in most of the countries for even the educated and the skilled persons. Unemployment among physicians in Iran has been the subject of discussion during the past so many years, and its severity, causes and ways of combating it has been a focus of discussions in different circles. It is a mail survey conducted on random sample, sized 5482 physicians, from registered physician roster at the Medical Association of Islamic Republic of Iran. The questionnaire included items on employment and unemployment quality and some related factors. The final analysis is based on the 2789 returned questionnaires. Total unemployment has a point prevalence of 9.4% among Iranian physicians [95% CI: 8.3% - 10.5%]. When summed with physicians with qualitative unemployment, this figure reaches 13.7% [95% CI: 12.4% - 14.9%]. Considering quantitative unemployment, the total ratio reaches 25.5%. Half of the unemployed physicians have seeked jobs for more than a year, and they mostly attribute their failure to low income of medical jobs and limited positions for physicians in the governmental sector. Unemployment, in different forms from total to qualitative and quantitative forms affects from one-tenth to one fourth of Iranian physicians. This problem is not limited to Iran, but has been seen in many other countries, especially those that had increased the strength of their medical students in the past. The solution of this problem needs its accurate identification and factors affecting it or related to it. Solving this problem needs finding its accurate fissure and the related factors